Figure 9.

An example of membrane protein (β₂AR) engineering toward successful crystallization. Removal of stabilizing point mutations (PDB 3D4S):²⁵⁶ (A) mutation E122^3.41W stabilizing the structure, (B) mutation N187E introduced to avoid glycosylation. The altered proteins are shown on the left panels and the native protein on the right. The spheres colored according to the atom type represent the native and mutated residues. The neighboring residues are shown in stick representation. A water molecule is shown as a ball-and-stick representation. Reproduced with permission from ref (255). Copyright 2015 American Chemical Society.