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. 2019 Jul 8;30(9):1573–1586. doi: 10.1681/ASN.2019010089

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Copyright © 2019 by the American Society of Nephrology

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Figure 5. — Expression of chemokines and recruitment of inflammatory cells is suppressed in Gprc5b-cKO glomeruli. (A) The expression of 21 chemokines is significantly downregulated in Gprc5b-cKO glomeruli as shown by a heat map generated from RNA sequencing data. (B) Validation of the downregulation of ccl2 expression by quantitative PCR. GAPDH was used as a housekeeping gene. **P<0.01. (C and D) Immunofluorescence analysis of CD45-positive cells in glomeruli 36 hours after LPS treatment shows significantly less immune cells in Gprc5b-cKO glomeruli when compared with controls. Magnifications: ×200. *P<0.05.