Figure 1.
Verification of Twist1 deficiency in peritoneal macrophages and UUO kidneys from MKO mice. (A) Twist1 mRNA expression in kidneys from sham and UUO mice (n=3). (B) Genotyping the mice by PCR analysis of genomic DNA. (C) Verification of macrophage-specific deletion of Twist1 in MKO mice. mRNA expression for Twist1 in isolated peritoneal macrophages (“Macro”), T cells, B cells, and in whole kidney and heart from WT and MKO animals (n=6). (D) Twist1 mRNA expression in kidneys from WT and MKO mice after UUO (n≥7). Data represent the mean±SEM. *P<0.05, **P<0.01, ***P<0.001. fl, floxed; UUO, unilateral ureteral obstruction; MKO, LysM-Cre; Twist1fl/fl; WT, wild type.