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. 2019 Jul 17;30(9):1674–1685. doi: 10.1681/ASN.2018121253

Figure 2.

Figure 2.

Twist1 deletion in macrophages augments collagen deposition in mice after ureteral obstruction. (A) Hydroxyproline content from obstructed WT and MKO kidneys after UUO at 2 weeks (n≥7). (B) Representative sections from obstructed WT and MKO kidneys stained with Col-I. Scale bar, 100 μm (C) Western blot for Col-I, α-SMA, Col-IV and FN in kidneys from WT and MKO mice after UUO at 2 weeks. (D) Semiquantitative determination of Col-I, α-SMA, Col-IV, and FN protein in the kidneys from WT and MKO mice after UUO (n≥7). (E) mRNA levels of inflammatory cytokines in obstructed kidneys from WT and MKO mice (n≥7). (F) mRNA levels of fibrosis markers/mediators in obstructed kidneys from WT and MKO mice (n≥7). Data represent the mean±SEM. *P<0.05, **P<0.01, ***P<0.001. Col-I, collagen-I; FN, fibronectin; α-SMA, α-smooth muscle actin; UUO, unilateral ureteral obstruction; MKO, LysM-Cre; Twist1fl/fl; WT, wild type.