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. 2019 Jul 17;30(9):1674–1685. doi: 10.1681/ASN.2018121253

Figure 5.

Figure 5.

Twist1-deficient peritoneal macrophages (MKO) have blunted induction of MMP13 after in vitro activation by IFNγ plus LPS treatment. (A) mRNA levels of MMP13 in WT versus MKO macrophages from groups as indicated. (B) Western blots of MMP13 protein in WT versus MKO macrophages with collagen-zymographic analysis of MMP13 activity in macrophage culture supernatants shown on bottom. (C) Determination of MMP13 protein levels from B using densitometry (n=3). (D) Zymographic analysis of relative MMP13 activity levels in supernatants from WT versus MKO macrophage cultures (n=3). Data represent the mean±SEM. *P<0.05, **P<0.01, ***P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP13, matrix metallopeptidase 13; MKO, LysM-Cre; Twist1fl/fl; WT, wild type.