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. 2019 Aug 29;13:928. doi: 10.3389/fnins.2019.00928

FIGURE 2.

FIGURE 2

Effect of cuneate nucleus (CN) lesions on the inhibition of morphine or ethanol self-administration behaviors by acupuncture at HT7. (A–D) The effect of CN lesions on the inhibition of morphine self-administration behaviors by acupuncture at HT7. A schematic of the procedure of the morphine self-administration experiment (A). While sham operation without HT7 acupuncture did not affect the number of active lever presses (Con) (C1), the active lever response in the HT7-treated group was significantly reduced after HT7 stimulation compared to baseline (HT7; paired t-test, ∗p = 0.008 vs. Baseline) (C2). These acupuncture effects were ablated in the rats with CN injury (CN X+HT7) (C3). HT7 acupuncture significantly reduced morphine intake in sham group, compared to baseline (Sham+HT7; paired t-test, ∗p = 0.017 vs. Baseline) (C4). There were no differences in the number of inactive lever presses among the groups or before and after HT7 (D1–D4). A representative toluidine blue-stained image of a CN lesion (B). (E–H) The effect of cuneate nucleus (CN) lesions on the inhibition of ethanol self-administration behaviors by acupuncture at HT7. A schematic of the procedure of the ethanol self-administration experiment (E). The sham operation without HT7 acupuncture did not affect the number of active lever presses (Con) (G1). The numbers of active lever presses in the HT7-treated group was significantly reduced after HT7 stimulation compared to baseline (HT7; paired t-test, ∗p = 0.042 vs. Baseline) (G2). This effect was not seen in the rats with CN injury (CN X+HT7; G3). There were no differences in the number of inactive lever presses among the groups or before and after HT7 (H1–H3). A representative toluidine blue-stained images of a CN lesion (F).