FIG. 5.

Inverse relationship between phosphatase and tensin homologue (PTEN) activity and prosurvival kinase (p-Akt) expression after a cervical hemi-contusion spinal cord injury (SCI). (A) The PTEN phosphorylation was considerably reduced by day three post-SCI, indicating PTEN phosphatase activity increased over this period. (B) Conversely, Akt phosphorylation significantly decreased starting at day one post-SCI. (C) Representative Western blots of PTEN, p-PTEN, p-Akt, and Akt. β-tubulin served as a loading control. Data were expressed as mean ± standard error of the mean; n = 4–5/time point; All data analyzed via one-way analysis of variance; *p < 0.05; **p < 0.01; ***p < 0.001.