FIG. 6.

Ventral horn motor neuron prosurvival kinase (p-Akt) expression mirrored total spinal tissue p-Akt expression post-spinal cord injury (SCI). (A) Illustration of region of neuronal imaging and quantification. (B) As shown for total spinal tissue, Akt phosphorylation decreased in ventral horn neurons throughout the first week post-SCI. (C) Ventral horn neurons were significantly decreased in the penumbral region beginning one day post-injury, but sustained this level throughout the first week after SCI. (D) Representative double immunofluorescence labeling shows decreased Akt phosphorylation in the ventral horn motor neurons over time post-injury. Data were expressed as mean ± standard error of the mean; n = 3/ time point; All data analyzed via one-way analysis of variance; *p < 0.05 compared with sham.