Fig. 6.

Differentiation of male and female ES cells into cardiac precursors. Top, images resulting from differentiation of ES cell lines according to standard protocol, with beating cardiac precursor cells at day 13 of LIF withdrawal. Below left, comparison of up-regulated genes between XX and XY ES cell lines indicating common and sex-specifically expressed RNAs (q < 0.01). Below right, expression of a subset of sex-biased genes expressed before and after differentiation of ES cells as analyzed by qRT-PCR in undifferentiated ES cells (gray) and derived cardiac precursors (coral). Error bars represent S.E.M. of duplicate experiments with three replicates each