Table 1. Transformation of 3-Phenyl Propionic Acid and Derivatives Thereof Using the Designed Oxidase–Peroxygenase Mutual Benefit System.
substrate | conv. (%) | %-ee(R) of diol 2 |
---|---|---|
1a | 92 | 97 |
1b | 74 | 59 |
1c | 93 | 98 |
1d | 76 | 92 |
1e | 94 | 98 |
1f | 95 | 95 |
1g | 79 | 95 |
1h | 97 | 93 |
1i | 88 | 91 |
1j | 36 | 25 |
Standard reaction condition: 50 mM PBS buffer (pH = 8.0), ammonium formate (100 mM), NADP+ (0.8 mM), NaCl (300 mM), substrate (5 mM), OleTJE (20 mg/mL), BM3-ANEH (10 mg/mL); total volume: 500 μL.