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. 2019 Mar 28;141(14):5655–5658. doi: 10.1021/jacs.9b01939

Table 1. Transformation of 3-Phenyl Propionic Acid and Derivatives Thereof Using the Designed Oxidase–Peroxygenase Mutual Benefit System.

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substrate conv. (%) %-ee(R) of diol 2
1a 92 97
1b 74 59
1c 93 98
1d 76 92
1e 94 98
1f 95 95
1g 79 95
1h 97 93
1i 88 91
1j 36 25
a

Standard reaction condition: 50 mM PBS buffer (pH = 8.0), ammonium formate (100 mM), NADP+ (0.8 mM), NaCl (300 mM), substrate (5 mM), OleTJE (20 mg/mL), BM3-ANEH (10 mg/mL); total volume: 500 μL.