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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Anal Chem. 2019 Feb 6;91(4):2805–2812. doi: 10.1021/acs.analchem.8b04699

Figure 3.

Figure 3.

Native RPLC (nRPLC) of AbbVie model ADC with varying hydrophobicity of bonded phase, as denoted by the structures. Gradient conditions are A: 50 mM NH4OAc, pH 7, B: 50 mM NH4OAc, 50% IPA, pH 7, 0–100%B/40 min, 100%B/5 min, 100 μL/min, 30 °C. The same tentative labels as for the HIC chromatogram of Figure 2d are made due to the similarity.