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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Anal Chem. 2019 Feb 6;91(4):2805–2812. doi: 10.1021/acs.analchem.8b04699

Figure 4.

Figure 4.

(a) nRPLC of the AbbVie model ADC Ab095-PZ, with peaks labeled based on the mass spectra. Gradient: 0 to 4.5% IPA/water over 3 min, then 4.5 to 50% IPA/water over 20 min. Detection at 280 nm. (b) Raw mass spectra for peaks as labeled, with the molecular weight based on deconvoluted mass spectra for peak ID. The blue lines show that extra peaks are from overlap.