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HIC separation of (a) model ADC and (b) commercial ADC Brentuximab vedotin. The dashed lines illustrate that the greater hydrophobicity of the mAb itself for Brentuximab vedotin. Tosoh TSKgel Butyl-NPR, 4.6 × 35 mm, 2.5 μm. MPA: 1.5 M ammonium sulfate, 25 mM sodium phosphate pH 7.0; MPB: 25 mM sodium phosphate pH 7.0 with 25% IPA; flow rate: 0.8 mL/min; column temp: 25 °C.
