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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Anal Chem. 2019 Feb 6;91(4):2805–2812. doi: 10.1021/acs.analchem.8b04699

Table 1.

Summary of HPLC Columns and Their Applications Herein

column application mobile phase A; B
Thermo MabPac RP RPLC, reduced ADC H2O + 0.1% DFA; ACN + 0.1% DFA
Supelco Bioshell A400 RPLC, nonreduced ADC H2O + 0.1% FA + 0.015% TFA; ACN + 0.1% FA + 0.015% TFA
Thermo-HIC butyl HIC, ADC (Purdue) 50 mM Na3PO4 + 1 M (NH4)2SO4, pH 7; 50 mM Na3PO4 + 30% IPA, pH 7
Tosoh TSKgel Butyl HIC, ADC (AbbVie) 25 mM Na3PO4 + 1.5 M (NH4)2SO4 pH 7; 25 mM Na3PO4 + 25% IPA, pH 7
PMMA, nonporous nRPLC, ADC 50 mM NH4OAc, pH 7; 50 mM NH4OAc + 50% IPA, pH 7