FIGURE 3:

Gain and loss of other domains among the Sec7 domain-containing subfamilies in eukaryotes. (A) The tree of eukaryotes depicting the ARF GEF subfamilies and domains present in the LECA, as well as gains and losses of additional domains and subfamilies. To ensure confidence in the predictions, losses are only proposed when two instances of loss have occurred in the relevant lineage. The open symbol depiction of the ARCC and TBS and their corresponding Ank-repeat and TBC-N domains, respectively, depicts putative origins of these families in the haptophyte E. huxleyi, alveolata, and amoebozoa. Phylogenetic analyses point toward the convergent evolution of these families with the acquisition of Ank repeats in amoebozoa, haptophytes and alveolates, and TBC-N domain in haptophytes and alveolates independently. (B) Gain and loss of ARF GEF subfamilies and domains in opisthokonts. The symbol legend is shown in the inset in B.