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. 2019 Jul 15;30(15):1834–1845. doi: 10.1091/mbc.E19-03-0139

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© 2019 Patel-King, Sakato-Antonku, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 4: — wdr92-1 mutant cells lack cilia. Left, differential interference contrast (DIC) images of control (cc4533) and wdr92-1 cells. The mutant lacks obvious ciliary structures. Right, immunofluorescence micrographs of cc4533 and wdr92-1 cells probed with monoclonal antibody 1869A that specifically recognizes outer arm dynein IC2 (King et al., 1985). No obvious staining of ciliary structures or stubs was observed. Scale bars: 5 μm.