FIGURE 4:

Reversion of eIF2α phosphorylation induced by menadione did not rescue protein synthesis. Incorporation of [³⁵S]-labeled amino acids in HEK293 cells. Total cell extracts were separated by SDS–PAGE and analyzed by autoradiography or immunodecorated with specific antibodies. (A) HEK293 cells were treated with menadione for 2 and 3 h with the GCN2 kinase inhibitor A92 in the presence of menadione (2 h) as indicated. (B) HEK293 cells were treated with menadione for 2 and 3 h with the PERK kinase inhibitor GSK2606414 in the presence of menadione (2 h) as indicated. Men, menadione.