FIGURE 8:
The integrated stress response is inhibited in NDUFA11-deficient HEK293T cells. (A) Incorporation of [35S]-labeled amino acids in HEK293T and HEK293T NDUFA11 knockout cells. Total cell extracts were separated by SDS–PAGE and analyzed by autoradiography or immunodecorated with specific antibodies to assess the phosphorylation of S6K1 (Thr389), 4E-BP1 (Ser65), and eIF2α (Ser51) proteins and ATF4 expression. The graphs show the quantification of three biological replicates of eIF2α phosphorylation and ATF4 expression. Mean ± SEM. (B) ATF4 mRNA levels in HEK293T and HEK293T NDUFA11 knockout cells. Mean ± SEM. n = 3. ***p < 0.001 by Student’s t test.