TABLE 1:
Microtubule sliding velocities in control buffer or buffer with the indicated purified expressed protein added.
Strain | Condition | µm/s ± SEM | n | p valuea |
---|---|---|---|---|
WT | Control | 17.10 ± 0.43 | 114 | |
pf18 | Control | 13.16 ± 0.40 | 108 | |
+His-PACRG | 13.51 ± 0.37 | 102 | p = 0.52 | |
+His-FAP20 | 13.84 ± 0.39 | 105 | p = 0.22 | |
+His-PACRG/-FAP20 | 13.91 ± 0.55 | 68 | p = 0.27 | |
pacrg (pf12) | Control | 14.13 ± 0.28 | 130 | |
+His-PACRG | 17.29 ± 0.41 | 131 | p < 0.0001 | |
+His-FAP20 | 13.37 ± 0.50 | 70 | p = 0.19 | |
+His-PACRG/-FAP20 | 16.18 ± 0.62 | 69 | p = 0.004 | |
pacrgR | Control | 16.96 ± 0.41 | 103 | p < 0.0001 |
fap20 (RL11) | Control | 13.95 ± 0.30 | 123 | |
+His-PACRG | 13.56 ± 0.42 | 68 | p = 0.45 | |
+His-FAP20 | 17.78 ± 0.37 | 116 | p < 0.0001 | |
+His-PACRG/-FAP20 | 18.10 ± 0.43 | 69 | p < 0.0001 | |
fap20R | Control | 17.09 ± 0.62 | 85 | p < 0.0001 |
pacrg; fap20 | Control | 13.40 ± 0.36 | 98 | |
+His-PACRG | 13.60 ± 0.41 | 95 | p = 0.71 | |
+His-FAP20 | 15.23 ± 0.36 | 133 | p = 0.0004 | |
+His-PACRG/-FAP20 | 17.73 ± 0.36 | 131 | p < 0.0001 |
aStudents’s t test was used to determine the p value when comparing a condition’s velocity to the control velocity of that same strain.