FIGURE 6:

Expression of key stem cell regulators, SYGL-1 and LST-1, in the distal gonad. SYGL-1 and LST-1 proteins were V5-tagged and visualized with a V5 antibody, as reported previously for hermaphrodites (Shin, Haupt, et al., 2017). (A) Immunostaining of male distal gonads: SYGL-1 (magenta, left panels) and LST-1 (yellow, right panels). Top panels: single channel maximum intensity confocal projections. Middle panels: single channel, single confocal slice through the middle of gonad. Bottom panels: same as the middle panel, merged with DAPI. *Distal end. (B) ImageJ quantitation of z projections of stained gonads, all done in parallel. Expression in males (blue); expression in hermaphrodites (gray) (n = 9 for both SYGL-1 and LST-1 males; n = 6 for both SYGL-1 and LST-1 hermaphrodites; n = 3 for both control males and hermaphrodites). Dashed lines indicate the average position where expression dropped to a background level when scored by eye. (C) Number of germ cells positive for LST-1 or SYGL-1 in male or hermaphrodite germlines.