FIGURE 7:

The sygl-1 and lst-1 mRNA and protein expression in whole gonads. (A–C) Projected confocal z stacks of adult male (left) or hermaphrodite (right) entire gonads. An asterisk marks the distal end; PZ arrowhead marks proximal boundary of the PZ. (A) The sygl-1 smFISH. The sygl-1 transcripts are limited to the distal end of male gonads (this work), but in hermaphrodites, they are found both distally and proximally, as reported before (Kershner et al., 2014; Lee et al., 2016). (B) SYGL-1 immunostaining. SYGL-1 protein is limited to the distal end of male gonads (this work), but in hermaphrodites, SYGL-1 is found both distally and again proximally, as reported before (Shin, Haupt, et al., 2017). (C) LST-1 immunostaining. Male germlines express LST-1 protein in a restricted region in the distal germline and also more proximally. LST-1 is enriched in perinuclear granules distally (Figure 6A), but is less granular proximally (inset). Inset is taken from a single confocal slice in the area indicated by the yellow dotted box in the full germline image. In hermaphrodites, LST-1 expression is strong distally and weak proximally, as reported before (Shin, Haupt, et al., 2017). *Distal end.