Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 1;30(14):1716–1728. doi: 10.1091/mbc.E18-12-0811

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Gravotta et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 10: — Compartmental distribution of γ1 and γ2 studied by quantitative immunofluorescence colocalization. Wild-type MDCK cells were fixed and processed for immunofluorescence using a mouse antibody against γ1 adaptin and a rabbit antibody against γ2 adaptin and different antibodies against TGN (TGN38) and ARE (Rab11) markers. Top panels display individual markers; bottom panels display pairs of colocalized markers. (A) Colocalization with TGN marker. Note areas of extensive colocalization between γ1, γ2, and TGN 38, as well as colocalization between γ1 and γ2. (B) Colocalization with CRE marker. For CRE labeling, cells were loaded with biotinylated transferrin for 90 min before fixation and processing for γ1 and γ2 immunofluorescence as described above. Subsequently, the cells are decorated with Alexa 647–streptavidin. (C) Colocalization with ARE marker. Cells are processed with antibody against γ1 adaptin and Rab 11, followed by the corresponding secondary antibodies. γ2 localization studies were prevented by lack of appropriate antibodies. Displayed images represent x-z projections of 15 slices. Note the apical localization of ARE with respect to the TGN and the extensive colocalization of γ1 and TGN. There is little colocalization of γ1 with ARE, likely reflecting projections of TGN fragments at that level. (D) Bar graph quantification of the colocalization of γ1 and γ2 with TGN, CRE, and ARE. (E) Bar graph quantification of the colocalization of TGN, CRE, and ARE with γ1 and γ2.