FIGURE 3:

Mutations in the predicted Snc1 JMH result in localization to the vacuole membrane. (A) Helical wheel representation of the predicted structural features of the nine-amino acid region required for retrograde trafficking of Snc1. Basic residues are colored blue and hydrophobic residues are colored gray. Note the amphipathic nature of this segment, with charged residues on one face. (B) Micrographs of wild-type cells expressing GFP-Snc1-TF4 truncation mutant containing indicated point mutations in the JMH. (C) Micrographs of wild-type and snx4Δ cells expressing full-length GFP-Snc1 containing the indicated point mutations in the predicted JMH. An approximate medial single Z section is shown unless otherwise indicated. Scale bars indicate 5 μm.