FIGURE 4:

Blebbistatin reduces migration but rescues nuclear damage. (A) Number of migrated C2C12 myoblasts—treated or not with blebbistatin—on the bottom of a 3- or 8-μm pore transwell membrane per 0.12-mm² field. Blebbistatin reduces migration rate regardless of pore size. N = 5–10 samples. (B) Nuclear bleb formation after 3-μm pore migration is significantly reduced by blebbistatin treatment. N = 80–351 cells. (C) Number of γH2AX foci per cell, normalized to top of membranes without blebbistatin. Blebbistatin treatment has no effect on DNA damage level without migration. DNA damage increases with constricted migration through 3-μm pores, but blebbistatin treatment significantly reduces this excess damage. N = 80–216 cells. * indicates significant (p < 0.05) difference from 8-μm migration or † from migration with blebbistatin.