Movie S2.

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Simulation of elongating invagination with force threshold decreasing with time. Left panel: 3D distribution of actin density (color) in the vicinity of the tubule. Middle panel: actin density and its velocities (arrows, scale bar in upper-left corner of the middle panel corresponds to 0.08 μm/s) are shown for r-z sections of 3D geometry. Right panel: deformable computational mesh used in simulation; deformations of mesh conform to moving invagination. The movie was made with a color code built into COMSOL, which is slightly different from that used in the Figures.