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. 2019 Jul 3;213(1):195–211. doi: 10.1534/genetics.119.302363

Figure 2.

Figure 2

PEF1-GFP accumulates at the contact point of fusing cells undergoing lysis. (A) Time course of two germlings undergoing mutual attraction and fusion. At time point 6 min, note the formation of a membrane protuberance (arrow) followed by rapid vacuolization of both fusion partners indicating lysis. Time = min. (B) PEF1-GFP localization during successful cell fusion (strain GN9-22, Ptef-1-pef-1-gfp). (C) PEF1-GFP accumulation at the contact point of a fusion pair undergoing lysis (arrow) (strain GN9-22, Ptef-1-pef-1-gfp). (B and C) Top images: DIC; bottom images: GFP fluorescence. (D) Quantification of PEF1-GFP recruitment in healthy and lysed cell pairs of strains GN9-22 (∆pef1, Ptef-1-pef1-gfp) and GN3-9 (∆Prm1, Pccg-1-pef1-gfp). Error bars indicate the SD calculated from three independent experiments (n = 100 each). Note that while the percentage of lysed cell pairs showing PEF1-GFP accumulation at the fusion point is comparable for both strains, the overall number of lysed cell pairs is significantly higher in ΔPrm-1 compared to the wild-type background strain (33 lysed pairs out of 300 total, compared to 8 out of 300 total, respectively). Bar, 5 µm.