Figure 5.

PEF1-GFP is recruited in response to nystatin-induced cell lysis. (A and B) Subcellular localization of PEF1-GFP in germlings of strain GN9-22 (Ptef-1-pef-1-gfp). (A) Control, without the addition of nystatin. (B) Nystatin was added directly after the photo for time point 0’ was taken. (A and B) DIC image on the left: 0 sec; DIC image on the right: 120 sec. Bar, 5 µm. Arrows in B indicate punctate PEF1-GFP aggregates. (C) Quantification of cells exhibiting PEF1-GFP recruitment to the plasma membrane after treatment with nystatin on MM and MM without Ca²⁺. Error bars indicate the SD calculated from three independent experiments (n = 100 each). The asterisks represent statistically significant differences determined by the Student’s t-test (*P ≤ 0.05; ***P ≤ 0.001).