Table 3. Differentially expressed proteins of E. cloacae represented as fold changes in response to cadmium treatment.

Proteins were identified by QTOF mass spectrometry.

Spot no.	Accession no.	Protein names	Fold change ATCC 13047 + Cd	Fold change EC01 + Cd	Fold change EC07 + Cd	Anova	M.W.	pI	Score	% protein seq. coverage	Matched peptide
1	KGB06270.1|	Catalase HPII	−1.07	1.76	−1.11	3.42E−03	83,514	5.63	331	14	28(14)
2	KGB06270.1|	Catalase HPII	2.23	2.33	−1.04	2.89E−04	83,514	5.63	314	7	13(9)
3	KGB06270.1|	Catalase HPII	−1.15	1.34	1.31	5.33E−01	83,514	5.63	1,587	16	79(67)
4	KGB06270.1|	Catalase HPII	1.57	1.14	1.21	1.02E−05	83,514	5.63	1,396	22	76(63)
5	KJC00120.1|	Hydroperoxidase	−1.56	−1.59	−2.04	1.70E−07	83,488	5.66	298	13	24(14)
6	CZY07068.1|	Hydroperoxidase II	Undetectable	−1.85	Undetectable	4.33E−02	83,493	5.76	151	7	9(7)
7	SAG51425.1|	Hydroperoxidase II	Undetectable	−1.06	Undetectable	1.52E−02	83,473	5.61	1,471	24	85(71)
8	KJC00120.1|	Hydroperoxidase II	Undetectable	Undetectable	−1.87	2.82E−02	83,488	5.66	791	18	43(35)
9	KJX08087.1|	Succinate-semialdehyde dehydrogenase	1.22	−1.06	−1.13	7.38E−10	49,930	5.71	516	25	89(34)
10	KTI00918.1|	Dihydrolipoamide dehydrogenase	−1.03	1.46	−1.05	1.66E−06	50,867	5.79	302	16	12(10)
11	KJC00120.1|	Hydroperoxidase	Undetectable	Undetectable	3.88	3.11E−04	83,488	5.66	580	20	34(27)
12	ADF60390.1|	Chaperone protein DnaK	−1.38	−1.06	−1.98	1.39E−01	69,092	4.82	120	13	13(8)
13	SAB00249.1|	30S ribosomal protein S1	−2.14	−1.28	−2.04	1.40E−01	61,289	4.89	134	14	23(8)
14	KLG03309.1|	Peroxiredoxin OsmC	−7.07	−2.44	−1.15	6.58E−03	15,005	6.04	217	52	52(21)
15	KGB03490.1|	DNA protection during starvation protein	−7.51	−3.74	−1.01	1.39E−02	18,720	5.94	1,022	76	205(84)
16	KPU05892.1|	Superoxide dismutase	1.26	−1.16	−1.23	2.94E−02	17,768	6.43	766	33	66(33)
17	ADF61100.1|	Type VI secretion system secreted protein Hcp	Not analysis	Not analysis	Not analysis	Not analysis	16,996	5.76	432	40	25(17)
18	SAH00153.1|	OsmC family protein	Undetectable	Undetectable	−1.91	5.04E−08	15,149	5.74	156	52	18(13)
19	SAG00330.1|	DNA protection during starvation protein	Undetectable	Undetectable	2.01	4.55E−05	18,677	5.72	819	36	126(60)
20	SAG00330.1|	DNA protection during starvation protein	Undetectable	Undetectable	−5.1	4.68E−07	18,677	5.72	506	38	24(21)
21	KJQ00352.1	50S ribosomal protein L21	−1.33	−1.09	1.1	6.97E−06	11,543	9.85	58	7	1(1)
22	EUM00092.1|	Universal stress protein A	−1.84	−1.15	−1.38	7.28E−05	16,200	4.98	53	23	4(3)
23	AQT90079.1|	Outer membrane protein OmpX	−1.28	−1.22	1.3	1.35E−07	18,654	6.23	1,064	22	59(36)
24	SAF00184.1|	Protein YciE	Undetectable	Undetectable	−1.74	2.68E−04	19,036	4.85	173	28	12(10)
25	SAF00211.1|	Protein YciF	Undetectable	Undetectable	−1.06	4.32E−09	18,313	5.04	1,838	29	187(130)
26	OUF00075.1|	Osmotically-inducible protein Y	Not analysis	Not analysis	1.1	3.72E−03	21,426	6.17	1,181	35	53(44)
27	CZX11689.1|	Protein YciF	Undetectable	Undetectable	2.45	3.65E−05	18,325	4.95	535	60	138(35)
28	KPU03204.1|	Heat-shock protein Hsp20	1.3	2.39	1.29	6.21E−05	21,493	5.32	687	48	66(36)
29	SAD00058.1|	TrpR binding protein WrbA	−1.31	−1.01	1.41	8.72E−10	20,847	5.78	255	25	14(12)
30	KJX08128.1|	Periplasmic protein	−1.04	−1.78	−1.75	1.69E−05	21,374	8.63	1,272	54	73(51)
31	CZW02624.1|	Periplasmic binding protein/LacI transcriptional regulator	−2.05	1.011	−1.34	6.04E−09	35,617	5.88	1,576	53	136(88)
32	OUF11927.1|	Malate dehydrogenase	1.32	1.43	1.05	1.17E−09	32,673	5.9	1,086	42	55(42)
33	CZX00043.1|	2,5-diketo-D-gluconate reductase A	−1.09	1.21	−1.07	1.46E−02	30,934	5.49	219	54	42(19)
34	SAD00983.1|	Cationic amino acid ABC transporter substrate-binding protein	−1.37	−1.27	−1.45	4.27E−01	28,179	7.6	333	23	24(16)
35	KGB04009.1|	Bacterial extracellular solute-bindings, 3 family protein	−1.49	−1.91	1.14	6.37E−04	33,362	8.8	565	42	51(28)
36	KJC00085.1|	Glyceraldehyde-3-phosphate dehydrogenase	1.37	1.45	1.06	1.32E−02	35,648	6.61	585	42	169(58)
37	SAJ08363.1|	Glyceraldehyde-3-phosphate dehydrogenase	1.62	1.72	1.07	5.94E−06	35,650	6.61	189	17	21(19)
38	ADF61881.1|	Superoxide dismutase	1.59	−1.05	1.17	1.01E−02	21,457	5.54	46	17	119(4)
39	SAD00058.1|	TrpR binding protein WrbA	−1.35	−1.7	−1.06	5.17E−02	20,847	5.78	1,160	35	51(40)
40	KTI00016.1|	Superoxide dismutase	−1.08	−1.05	1.19	4.00E−01	22,995	6.23	204	13	21(12)
41	KTI01411.1|	Hypothetical protein ASV14_00320	−1.21	−1.17	1.17	2.53E−07	37,677	5.33	1,254	47	88(54)
42	CZW04458.1|	Outer membrane protein A	−1.14	−1.43	1.24	4.62E−08	37,577	5.2	1,791	48	122(74)
43	KJX05875.1|	Porin [Enterobacter cloacae subsp. cloacae]	−1.36	−1.26	1.27	5.81E−09	39,231	4.58	493	32	44(22)
44	5FVN|A	Chain A, X-ray Crystal Structure of Enterobacter cloacae Ompe36 Porin	−1.28	1.06	1.09	2.01E−07	37,857	4.39	3,805	35	155(133)
45	SAF58867.1|	OmpC porin	−1.24	−2.06	1.38	8.59E−07	39,850	4.44	573	35	78(35)
46	KJQ00573.1|	Fructose-bisphosphate aldolase	1.5	1.6	−1.18	3.50E−03	39,384	5.52	124	23	14(4)
47	SAH00347.1|	Alcohol dehydrogenase	−1.25	1	−1.15	2.21E−05	35,832	5.7	1,725	60	231(109)
48	KPU01695.1|	Elongation factor Tu	−1.57	−1.55	1.12	2.09E−03	43,460	5.25	494	27	101(32)
49	KPU01695.1|	Elongation factor Tu	−1.59	−1.17	−1.46	4.33E−02	43,460	5.25	481	29	28(19)
50	CZW60501.1|	Isocitrate dehydrogenase	−1.36	1.17	−1.25	9.02E−03	46,145	5.14	127	23	17(8)
51	CZW60501.1|	Isocitrate dehydrogenase	−1.18	1.18	−1.51	1.49E−08	46,145	5.14	79	9	4(3)
52	SAH00247.1|	Enolase	1.19	1.56	−1.35	1.65E−03	45,632	5.19	85	10	13(6)
53	SAI00778.1|	Elongation factor Tu	−1.39	1.25	−1.8	3.38E−02	43,444	5.25	673	33	50(32)
54	WP_075208257.1|	Translation elongation factor Tu	−1.37	1.04	−1.84	2.35E−05	40,036	5.01	495	40	36(24)
55	KJJ00122.1|	Molecular chaperone GroEL	2.29	2.51	−1.29	7.48E−06	57,205	4.88	800	38	48(40)
56	KPU06091.1	Molecular chaperone GroEL	1.32	1.33	−1.09	3.85E−06	57,209	4.85	484	36	43(32)
57	SAB11354.1|	Domain of Uncharacterised Function (DUF326)	Undetectable	1.35	Undetectable	9.38E−11	13,306	5.27	3,402	66	123(110)
58	CZV11460.1|	Putative hydrolase	1.59	−1.1	−1.24	1.98E−02	72,227	5.28	213	10	16(10)
59	KPU06091.1|	Molecular chaperone GroEL	1.85	1.36	1.09	1.56E−02	57,209	4.85	800	27	69(58)
60	KJJ00126.1|	Aspartate ammonia-lyase	−1.59	1.55	−1.37	9.13E−06	52,776	5.05	140	23	46(14)
61	SAB00249.1|	30S ribosomal protein S1	−4.92	−2.24	1.03	2.31E−03	61,289	4.89	139	10	25(12)
62	SAJ00217.1|	F0F1 ATP synthase subunit beta	1.29	1.29	−1.14	2.46E−04	50,268	4.89	305	19	113(30)
63	CZY00919.1|	Maltoporin	−1.25	1.08	1.21	1.38E−04	48,457	4.79	471	27	58(22)
64	SAB00172.1|	Tellurium resistance protein TerE	−1.75	−1.66	−1.27	1.60E−02	20,437	4.69	688	30	52(32)
65	SAB00191.1|	Tellurium resistance protein TerD	−1.1	−1.61	−1.28	3.66E−05	20,573	4.64	1,803	36	58(57)
66	KTI00836.1|	Molecular chaperone DnaK	−1.73	−1.61	1.22	4.85E−04	69,092	4.82	473	9	43(20)
67	WP_004144995.1|	MULTISPECIES: ATP synthase subunit alpha	2.98	1.24	−1.19	4.42E−03	55,363	5.73	99	19	23(8)
68	CZZ00122.1|	Glucose-specific PTS system component	Undetectable	Undetectable	2.16	1.38E−01	18,313	5.04	104	7	3(3)
69	CZZ00122.1|	Glucose-specific PTS system component	−3.03	−1.71	−1.61	6.63E−05	18,222	4.73	660	27	32(23)
70	KGZ01983.1|	Chemical-damaging agent resistance protein C note like to terD domain protein and Tellurium resistance protein TerE	−3.64	−1.5	1.02	1.71E−01	20,463	4.69	392	39	56(25)
71	SAF00152.1|	Manganese catalase	Undetectable	Undetectable	1.13	3.21E−05	31,431	4.8	338	20	41(13)
72	KJJ00123.1|	Molecular chaperone GroES	−1.05	1.11	1.27	1.02E−05	10,354	5.38	83	27	9(7)
73	KJJ00123.1|	Molecular chaperone GroES	Undetectable	−1.13	1.29	4.92E−03	10,354	5.38	70	14	3(3)
74	KJJ00123.1|	Molecular chaperone GroES	−1.21	−1.32	−1.25	1.44E−01	10,354	5.38	555	58	71(36)
75	Q84FI1.1|DPS_ENTCL	RecName: Full = DNA protection during starvation protein	Undetectable	Undetectable	−1.21	8.29E−07	18,691	5.95	133	23	10(9)
76	SAC00082.1|	LysM domain/BON superfamily protein	−3.09	−1.52	Undetectable	5.26E−06	15,895	5.4	155	24	9(9)
77	KJC00085.1|	Glyceraldehyde-3-phosphate dehydrogenase	−3.26	−2.55	Undetectable	3.38E−07	35,648	6.61	227	16	47(15)
78	KPU04452.1|	Ecotin	−1.08	−1.56	Undetectable	1.79E−08	19,034	8.35	208	28	22(11)
79	ESM17978.1|	Glutamine-binding periplasmic protein	−1.03	2.38	1.8	1.04E−02	26,950	8.91	130	15	13(8)
80	KGB04009.1|	Bacterial extracellular solute-bindings, 3 family protein	Not analysis	Not analysis	Not analysis	Not analysis	33,362	8.8	884	19	55(52)