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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Curr Protoc Cell Biol. 2019 Sep;84(1):e89. doi: 10.1002/cpcb.89

Figure 2. Classical ChIP-seq method.

Figure 2

First, protein is crosslinked to DNA with formaldehyde. Sonication is then used to fragment chromatin. Protein-DNA complexes are immunoprecipitated. Crosslinking is reversed, and DNA is purified. DNA is then prepared for sequencing. Adapters are ligated to both 5’ and 3’ ends. Lastly, DNA is size selected and PCR amplified. Libraries are then ready to be sent for sequencing.