Table 5. Statistical summary of the candidate genes with the greatest potential to serve as a bioindicator of TH insufficiency.

All PTU doses represent the maternal dose administered. Positive values represent upregulation and negative downregulation. Values listed are the percent change in gene expression, 6 SEM. Resulting overall ANOVA F-statistics and p-values for each gene are shown. Mean contrast tests were then conducted using Dunnett’s t-test with a=0.05. Significant dose effects are indicated by asterisks.

	Experiment 1: GD20 forebrain
		0.1 ppm PTU	0.5 ppm PTU	1 ppm PTU	2 ppm PTU	3 ppm PTU	F statistic	p-value
Calcium Signaling - CREB pathway	Camk4	−7±8	8±6	−22±5	−31±5**	−43±5**	F(5,41)=9.07	<0.0001
Thyroid signaling	Thwb	34±13	38±14	−21±12	−38110	−51±6**	F(5,39)=10.10	<0.0001
	Experiment II: PN14 neocortex
		1 ppm PTU	2 ppm PTU	3 ppm PTU	10 ppm PTU		F statistic	p-value
Calcium signaling - GABAergenic neurons	Parv	−30±19**	−60±7**	−81±3**	−97±.6**		F(4,30)=49.49	<0.0001
	Colla2	−26±8**	−54±6**	−640**	−86±1**		F(4,30)=37.34	<0.0001
	Gjb6	−25±6**	−44±3**	−48±3**	−58±3**		F(4,30)=33.51	<0.0001
Cell and tissue structure	Itih3	−28±6**	−48±6**	−65±4**	−82±2**		F(4,30)=38.60	<0.0001
	Mog	−22±12	−46±14**	−71±7**	−90±3**		F(4,30)=13.63	<0.0001
	Agt	−26±10**	−53±7**	−71±4**	−85±1**		F(4,30)=32.49	<0.0001
	Pnoc	−13±2**	−23±3**	−32±4**	−55±2**		F(4,30)=48.62	<0.0001
Neuronal signaling and migration	Ngf	−10±7	−30±9**	−46±4**	−58±4**		F(4,30)=14.03	<0.0001
	Sema7a	−30±9	−60±7**	−81±3**	−97±.6**		F(4,30)=16.41	<0.0001
	Hod	−20±5**	−44±5**	−52±3**	−73±1**		F(4,30)=58.70	<0.0001
Cell differentiation and death	Hr	−17±8	−46±12**	−71±6**	−90±.5**		F(4,30)=27.39	<0.0001
	Kfl9	−30±9	−60±7**	−81±3**	−97±.6**		F(4,30)=45.72	<0.0001