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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Prog Nucl Magn Reson Spectrosc. 2019 May 11;112-113:55–102. doi: 10.1016/j.pnmrs.2019.05.002

Figure 26.

Figure 26.

Representative experimental off-resonance R RD profiles showing three-state exchange with “minor” exchange i.e., direct exchange between the two excited states[195]. Shown in solid lines are three-state fits to the experimental off-resonance R RD profiles (hpGT-GGC, pH 8.0, 10 °C) with and without direct “minor” exchange between the two excited states[195], with the inclusion of “minor” exchange fitting the data better. Spin-lock amplitudes are color-coded. Error bars represent experimental uncertainty determined based on a Monte-Carlo error propagation scheme (Section 6.3). The direction of initial alignment of the magnetization during B-M fitting was determined as described in Section 6.1, while taking into consideration the ES with the highest population. Reprinted by permission from [195].