Fig 5. DCs exposed to rω-1 induce a T_mix cell population less susceptible to SF162 (R5) but not LAI (X4) infection.

(A) Percentage of SF162 or LAI infected cells in T_mix cell populations induced by rω-1 unexposed DCs was set to 100% and the level of infection found in T_mix cell cultures induced by rω-1 exposed DCs was expressed as a percentage of this 100%. (B) The percentage of SF162⁺ cells found in T_mix cell populations induced by unexposed DCs versus the percentage found T_mix cell populations induced by rω-1 exposed DCs (left panel) with +/- SE shown, with the same shown for LAI (right panel) with +/- SE depict. (C) Average percentage (± SEM) of IL-4, IFN-γ and MIP-1β producing T-cells in T_mix cell cultures induced by unexposed or rω-1 exposed DCs and depict with SD. (D) Percentage of cells with high CCR5 expression on their surface as well as the quantity of CCR5 per cell (geometric mean) for T_mix cell cultures induced by unexposed or rω-1 exposed DCs (dotted line unstained control), representative figure of 3 independent experiments. (B, C) Data from four independent experiments (± SD) using four different donor combinations where DCs were matured in the presence of 2–4μg/ml rω-1. *p<0.05.