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. 2019 Sep 5;14(9):e0222089. doi: 10.1371/journal.pone.0222089

Table 2. Oligonucleotide primers used for HPV diagnosis, HPV16 typing, viral load quantitation (HPV16 URR) and E2: E6 PCR in the present study.

Primer Nucleotide Position in HPV Amplicon size (bp) Primers
Type
Primer sequences Final Conc.
(μM)
References
MY 09 and L1 Consensus 450 Forward 5’CGT CCM ARR GGA WAC TGATC-3’ 20 pmoles [39]
MY 11 Reverse 5’-GCM CAG GGW CAT AAY AAT GC-3’
(M = A+C, W = A+T, Y = C+T, R = A+G)
HPV16URR 7763–7781 217 Forward 5’-AAG GCC AAC TAA ATG TCA C-3’ 20 pmoles [37]
57–75 Reverse 5’-CTG CTT TTA TAC AA CCG G-3’
p53 Exon 5 463–482 184 Forward 5’-TAC TCC CCT GCC CTC AAC AA-3’ 20 pmoles [37]
534–562 Reverse 5’-CAT CGC TAT CTG AGC AGC GC-3’
HPV16 E2 2734~2753 1139 Forward 5'-AGG ACG AGG ACA AGG AAA A-3' 20 pmoles [37]
3853–3872 Reverse 5'-GGA TGC AGT ATC AAG ATT TG-3'
HPV 16 E6 83–102 477 Forward 5’-GAA ACC GGT TAG TAT AAA AGC AGA C-3’ 20 pmoles [38]
540–559 Reverse 5’- AGC TGG GTT TCT CTA CGT GTT CT-3’
β-Globin 268 Forward 5’-GAA GAG CCA AGG ACA GGT AC-3’ 10 pmoles [37]
Reverse 5’-CAA CTT CAT CCA CGT TAC ACC-3’