Table 2. Oligonucleotide primers used for HPV diagnosis, HPV16 typing, viral load quantitation (HPV16 URR) and E2: E6 PCR in the present study.
Primer | Nucleotide Position in HPV | Amplicon size (bp) | Primers Type |
Primer sequences | Final Conc. (μM) |
References |
---|---|---|---|---|---|---|
MY 09 and | L1 Consensus | 450 | Forward | 5’CGT CCM ARR GGA WAC TGATC-3’ | 20 pmoles | [39] |
MY 11 | Reverse | 5’-GCM CAG GGW CAT AAY AAT GC-3’ | ||||
(M = A+C, W = A+T, Y = C+T, R = A+G) | ||||||
HPV16URR | 7763–7781 | 217 | Forward | 5’-AAG GCC AAC TAA ATG TCA C-3’ | 20 pmoles | [37] |
57–75 | Reverse | 5’-CTG CTT TTA TAC AA CCG G-3’ | ||||
p53 Exon 5 | 463–482 | 184 | Forward | 5’-TAC TCC CCT GCC CTC AAC AA-3’ | 20 pmoles | [37] |
534–562 | Reverse | 5’-CAT CGC TAT CTG AGC AGC GC-3’ | ||||
HPV16 E2 | 2734~2753 | 1139 | Forward | 5'-AGG ACG AGG ACA AGG AAA A-3' | 20 pmoles | [37] |
3853–3872 | Reverse | 5'-GGA TGC AGT ATC AAG ATT TG-3' | ||||
HPV 16 E6 | 83–102 | 477 | Forward | 5’-GAA ACC GGT TAG TAT AAA AGC AGA C-3’ | 20 pmoles | [38] |
540–559 | Reverse | 5’- AGC TGG GTT TCT CTA CGT GTT CT-3’ | ||||
β-Globin | 268 | Forward | 5’-GAA GAG CCA AGG ACA GGT AC-3’ | 10 pmoles | [37] | |
Reverse | 5’-CAA CTT CAT CCA CGT TAC ACC-3’ |