Table 3. Optimization of primer concentrations for RT-LAMP assay for VT strains of Citrus tristeza virus.

Inner primers(FIP/BIP) (μM)	Outer primers(F3/B3) (μM)	Loop primers(LF/LB) (μM)	Time (min:sec)			MeanTime (min:sec)	SD
			R1	R2	R3
0.8	0.2	0.5	11:15	11:15	11:15	11:15	0.000
1.2			9:30	9:30	9:30	9:30	0.000
1.6			8:30	8:30	8:30	8:30	0.000
2			8:00	8:00	8:00	8:00	0.000
0.8		0.75	10:00	10:15	10:00	10:05	0.006
1.2			9:00	8:45	8:45	8:50	0.006
1.6			8:15	8:15	8:15	8:15	0.000
2			8:00	7:45	7:45	7:50	0.006
0.8		1	9:00	9:00	9:00	9:00	0.000
1.2			8:45	9:00	8:45	8:50	0.006
1.6			8:00	8:00	8:00	8:00	0.000
2			7:45	7:45	7:30	7:40	0.006