Table 3. Optimization of primer concentrations for RT-LAMP assay for VT strains of Citrus tristeza virus.
Inner primers(FIP/BIP) (μM) | Outer primers(F3/B3) (μM) | Loop primers(LF/LB) (μM) | Time (min:sec) | MeanTime (min:sec) | SD | ||
---|---|---|---|---|---|---|---|
R1 | R2 | R3 | |||||
0.8 | 0.2 | 0.5 | 11:15 | 11:15 | 11:15 | 11:15 | 0.000 |
1.2 | 9:30 | 9:30 | 9:30 | 9:30 | 0.000 | ||
1.6 | 8:30 | 8:30 | 8:30 | 8:30 | 0.000 | ||
2 | 8:00 | 8:00 | 8:00 | 8:00 | 0.000 | ||
0.8 | 0.75 | 10:00 | 10:15 | 10:00 | 10:05 | 0.006 | |
1.2 | 9:00 | 8:45 | 8:45 | 8:50 | 0.006 | ||
1.6 | 8:15 | 8:15 | 8:15 | 8:15 | 0.000 | ||
2 | 8:00 | 7:45 | 7:45 | 7:50 | 0.006 | ||
0.8 | 1 | 9:00 | 9:00 | 9:00 | 9:00 | 0.000 | |
1.2 | 8:45 | 9:00 | 8:45 | 8:50 | 0.006 | ||
1.6 | 8:00 | 8:00 | 8:00 | 8:00 | 0.000 | ||
2 | 7:45 | 7:45 | 7:30 | 7:40 | 0.006 |