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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: Cell Host Microbe. 2019 Aug 1;26(2):228–239.e8. doi: 10.1016/j.chom.2019.07.003

Fig 5. Ngo competence mutants resist killing by Nel DNA.

Fig 5.

CFUs of Ngo DNA uptake/transformation mutants incubated for 4 h with purified Nel DNA (20 μg/mL). WT: Ngo MS11. (A) ΔcomP: isogenic DNA uptake mutant deleted of the comP ORF; ΔcomP/comPWT: complemented ΔcomP strain. (B) ΔpilT: isogenic DNA uptake mutant deleted of the pilT ORF; ΔpilT/ipilT: ΔpilT complemented with WT pilT under control of the IPTG-inducible lac promoter, mock-induced with medium or induced with IPTG during overnight growth on agar and during the liquid killing assay. (C) N400: Ngo MS11 containing recA under control of the IPTG-inducible lac promoter (Tonjum et al., 1995) mock-induced with medium or induced with IPTG during overnight growth on agar and during the liquid killing assay. (A-C) Starting CFU: 5×105. n=3-4 (P<0.0005 WT vs ΔcomP; ΔcomP vs ΔcomP/comPWT; WT vs ΔpilT; uninduced ΔpilT/ipilT vs IPTG-induced ΔpilT/ipilT; WT vs mock-induced N400; mock-induced N400 vs IPTG-induced N400; One-way ANOVA with Tukey’s Multiple Comparison Test). LOD: 10 CFUs.