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. Author manuscript; available in PMC: 2019 Sep 5.
Published in final edited form as: Cell Rep. 2019 Aug 27;28(9):2317–2330.e8. doi: 10.1016/j.celrep.2019.07.063

Figure 1. PTEN Loss or Inactivation Upregulates PDHK1 in Cancer and Normal Cells.

Figure 1.

(A) Left: western blots showing PTEN and phospho-AKT expression in PTEN-deficient H1650 cancer cells stably expressing PTEN or empty vector (EV). Right: list of genes significantly upregulated (fold change > 2, multiple t tests, *p < 0.05, q < 0.2, n = 3 replicates) in GFP-expressing H1650 cells compared with H1650 cells stably re-expressing PTEN, by microarray analysis. Highlighted in red boxes are the top upregulated energy metabolism genes, including PDHK1, in H1650-GFP cells.

(B) Western blots showing PTEN, phospho-AKT, and PDHK1 expression in PTEN-deficient cancer cell lines stably expressing PTEN or empty vector (EV). (C and D) Same as (B) in PTEN-proficient cancer (C) or normal (non-cancer) (D) cell lines with stable PTEN knockdown. shPTEN, shRNA to PTEN; SC, scrambled control shRNA.

See also Figure S1 and Tables S1 and S2.