Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens - Ssb) | La | Addgene | RRID:Addgene_38241 | N/A |
| Antibody | anti-La (mouse monoclonal) | Origene Technologies | RRID: AB_2239866 | IF 1:100. WB: 1:1000 |
| Antibody | anti-Rab7 (goat polyclonal) | Santa Cruz | RRID: AB_2175486 | IF 1:100 |
| Antibody | anti-CD63 (mouse monoclonal) | Developmental Studies Hybridoma Bank | RRID: AB_528158 | IF 1:100 |
| Antibody | anti-CD9 (mouse monoclonal) | Biolegend | RRID: AB_314907 | IF 1:100 |
| Antibody | Anti-ITGA3 (rabbit polyclonal) | Abcam | RRID: AB_2810979 | WB 1:1000 |
| Antibody | anti-MFGE-8 (mouse monoclonal) | R and D systems | RRID: AB_2142466 | WB 1:1000 |
| Antibody | anti-Flot-2 (mouse monoclonal) | BD biosciences | RRID: AB_397766 | WB 1:1000 |
| Antibody | anti-Tsg101 (mouse monoclonal) | GeneTex | RRID: AB_373239 | WB 1:1000 |
| Antibody | anti-CD63 (mouse monoclonal) | BD biosciences | RRID: AB_396297 | WB 1:1000 |
| Antibody | anti-Ago2 (rabbit monoclonal) | Cell signaling | RRID: AB_2096291 | WB 1:1000 |
| Antibody | anti-Ago2 (rabbit monoclonal) | Abcam | RRID: AB_2713978 | WB 1:1000 |
| Antibody | anti-dicer (rabbit polyclonal) | Santa cruz | RRID: AB_639122 | WB 1:1000 |
| Antibody | anti-vinculin (rabbit monoclonal) | Abcam | RRID: AB_11144129 | WB 1:1000 |
| Antibody | anti-Rab27a (mouse monoclonal) | Abcam | RRID: AB_945112 | WB 1:1000 |
| Antibody | anti-nucleophosmin (mouse monoclonal) | Abcam | RRID: AB_297271 | WB 1:1000 |
| Antibody | anti-nucleolin (rabbit polyclonal) | Abcam | RRID: AB_776878 | WB 1:1000 |
| Antibody | anti-Hsc70 (rabbit monoclonal) | Abcam | RRID: AB_444764 | WB 1:1000 |
| Antibody | anti-tubulin (mouse monoclonal) | Abcam | RRID: AB_2241126 | WB 1:1000 |
| Cell line (Spodoptera frugiperda) | Sf9 | Other | N/A | Cell culture facility at UC Berkeley |
| Cell line (Homo sapiens) | MDA-MB-231 | Other | N/A | Cell culture facility at UC Berkeley |
| Cell line (Homo sapiens) | MDA-MB-231 Rab27a KO | This study | N/A | Obtained by CRISPR-Cas9 |
| Cell line (Homo sapiens) | MDA-MB-231 Rab35 KO | This study | N/A | Obtained by CRISPR-Cas9 |
| Recombinant DNA reagent | UCOE- EF1a-dCas9-BFP-KRAB (plasmid) | N/A | N/A | Dr. Jonathan Weissman (University of California, San Francisco) |
| Recombinant DNA reagent | EF1Alpha-puro-T2A-BFP (plasmid) | Addgene | RRID:Addgene_60955 | N/A |
| Software, algorithm | GO term finder | N/A | RRID:SCR_008870 | N/A |
| Software, algorithm | REVIGO | N/A | RRID:SCR_005825 | N/A |
| Software, algorithm | mirDeep | N/A | RRID:SCR_010829 | N/A |
| Software, algorithm | HISAT2 | N/A | RRID:SCR_015530 | N/A |
| Software, algorithm | Bowtie | N/A | RRID:SCR_005476 | N/A |
| Software, algorithm | Samtools | N/A | RRID:SCR_002105 | N/A |
| Software, algorithm | Bedtools | N/A | RRID:SCR_006646 | N/A |
| Peptide, recombinant protein (enzyme) | TGIRT-III Enzyme | InGex | N/A | See Materials and methods for further details. |