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. Author manuscript; available in PMC: 2020 Sep 4.
Published in final edited form as: Neuron. 2019 Jun 27;103(5):853–864.e4. doi: 10.1016/j.neuron.2019.06.003

Figure 6. Investigating the role of graded Ryk signaling in interneuron lineage specification.

Figure 6.

(A) Schematic diagram of the regulation of tetracycline trans-activator, tTA2S by varying doxcycyline added to medium during ES cell differentiation to interneuron. tTA2S activity regulates the graded expression of NLS-Ryk-ICD. After differentiation into interneurons, cells were transplanted by UBM at e13.5 and analyzed at P21 for SST+ and PV+ expression. (B) Doxycyline dose response curve was determined empirically in order to ascertain appropriate low and high doxycycline concentrations for transplant experiments. C) Results of transplantation studies where ES cells were differentiated in no, low, or high doxycycline concentrations. Error bars standard error of the mean (* denotes p<0.05; ** denotes p<0.01; *** denotes p<0.001).