Fig. 5.

Properties of the screened mutants. (A) Noscapine turnover (dark bars); coupling efficiency (light bars). (B–F) Product distribution percentages for metabolites 1, 2a, 2b, 3, and 5. (F) was generated from data for meconine (5) which shares a similar UV absorption spectrum with the other noscapine metabolites (Fig. S1) and is produced stoichiometrically with 4. The WT enzyme and F87A, R47 L/Y51 F, R47 L/Y51 F/F87A, GVQ, GAQ and A3D did not show detectable activity and are not included. The small amounts of double-oxidation products detected (Table S2; Fig. S2) are not shown. Three replicates were used for each enzyme mutant; error bars are ± 1 SE. Numerical results are tabulated in the Supplementary Material (Table S3).