Fig. 4.

pTK1 containing the rat GluR1 5′ UTR region is sensitive to RA-treatment of SH-SY5Y cells. SH-SY5Y^RARα cells were transfected with the indicated plasmids and with pRL-TK expressing Renilla luciferase as a reference. After transfection, cells were treated with 10-μM ATRA for 24 h before luciferase activities were measured. a The graph shows standardised firefly luciferase activity in SH-SY5Y ^RARα cells grown in the absence of doxycycline. Firefly luciferase activity was normalised with respect to Renilla luciferase activity and then standardised with respect to normalised luciferase activity in cells not treated with ATRA (−ATRA), which was set at 1. b) Luciferase activity in SH-SY5Y ^RARα cells treated with doxycycline. Shown are normalised, standardised luciferase activities determined as described for a. Shown are mean values of three biological replicates. Error bars indicate standard error of the mean (SEM) (**p ≤ 0.01, ***p ≤ 0.001, Student’s t test)