Fig. 3.

ALKBH5 is overexpressed in GC and colocalizes with NEAT1. a RT-PCR followed by RNA immunoprecipitation showed that NEAT1 undergoes m⁶A modification in various gastric cells. b ALKBH5 and NEAT1 are coexpressed in GC tissue. c, d ALKBH5 expression in SGC-7901, BGC-823, and GES-1 cells as determined by RT-PCR and western blot analysis. e ALKBH5 expression in GC tissue compared with paired adjacent tissue in 57 patients as determined by IHC. f Representative images of ALKBH5 staining in gastric tissue (a, overexpression in GC; b, low expression in GC; c, weak expression in adjacent tissue). g Immunofluorescence indicating that ALKBH5 interacts with NEAT1