Fig. 6.

EZH2 and NEAT1 affected GC invasion and metastasis. a The expression of EZH2 protein was affected by NEAT1 and ALKBH5. b The mRNA level of EZH2 was not affected by NEAT1 or ALKBH5. c, d GC cells transfected with si-EZH2 or NC were assessed by RT-PCR. U6 and β-actin were used as loading controls in RT-PCR and western blot analysis. e, f Transwell assays were used to evaluate the role of EZH2 in invasion in EZH2-knockdown and EZH2-knockdown + NEAT1-overexpressing GC cells. In all figures, × 100 magnification was used. g, h Scrape motility assays were monitored for 24 h in EZH2-knockdown and EZH2-knockdown + NEAT1-overexpressing GC cells. In all figures, × 100 magnification was used