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. 2019 Sep 5;9:12806. doi: 10.1038/s41598-019-49291-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram of the experimental setup used for lifetime imaging. The pulsed laser and NIR camera are computer-controlled by a pulse generator to allow a time series of the fluorescence decay images for lifetime imaging. Analysis of the attenuation rate of fluorescence decay images obtained by the system allowed for measurement of the fluorescence lifetime at each pixel.