Fig. 5.

Increased colon mucus levels and reduced intestinal permeability in Il36rn−/− mice. a Representative micrographs of distal colon sections from wt and Il36rn−/− mice stained with H&E and AB/PAS. Scale bar = 1 μm. b Representative micrographs of colon sections stained with AB/PAS with mucus layer (ML) indicated by red arrow. Scale bar = 20 μm. c, d Quantitation of average goblet cells per crypt (c) and mucus layer thickness (d) in distal colon sections (n = 6 per group, 10 crypts measured per sample). Data shown representative of three independent experiments. e Relative gene expression of Muc2 gene in the distal colon of wt and Il36rn−/− mice (n = 6 per group). f Fluorescein isothiocyanate (FITC)-dextran concentration in the serum of wt and Il36rn−/− mice on HFD for 12 weeks, 4 h after oral gavage (n = 4 and 6 per group). Data show means ± SEM. Statistical analysis using two-tailed unpaired student’s t-test. *p < 0.05, **p < 0.01. Source data are provided as a Source Data file