Fig. 6.

IL-36 signalling increases goblet cell numbers and Muc2 gene expression. a, b Representative micrographs of AB/PAS-stained sections (a) and average goblet cells per crypt quantitation (b) in the colon of wt and Il36rn−/− mice after (day 6) three consecutive injections (days 1, 2, 3) of rIL-36Ra or PBS control (n = 4 and 5 per group). Scale bar = 1 μm. c Relative Muc2 gene expression in wt colon explants either untreated or stimulated for 4 h with recombinant IL-36α (200 ng/ml). Data show means ± SEM. Statistical analysis using two-tailed unpaired student’s t-test. *p<0.05. Source data are provided as a Source Data file