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. 2019 Sep 5;10:3993. doi: 10.1038/s41467-019-12005-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Vangl2 asymmetry is not affected in hair cells of MZwnt11 (wnt11f1) mutants. a–d Double β-II-Spectrin (labeling the cuticular plates) and Vangl2 immunodetection in primI neuromasts of 5 dpf wild type (a), MZscrib mutants (b), scrib siblings (c) and MZwnt11 (wnt11f1) mutants (d). e–h Double β-II-Spectrin (labeling the cuticular plates) and Vangl2 immunodetection in primII neuromasts of 5 dpf wild type (e), MZscrib mutants (f), scrib siblings (g) and MZwnt11 (wnt11f1) mutants (h). i Quantification of the percentage of hair cells that show asymmetric Vangl2 for each of the conditions in both primordia-derived neuromasts. j–n Clonal localization of GFP-XVangl2 in hair cells of neuromasts that show primI and primII polarity in wild type (j, k) and MZwnt11 (wnt11f1) mutants (m, n) neuromasts. The majority of the clones were localized in neuromasts in the head, which show the same hair cell orientation as in primI and primII-derived neuromasts in each of the cases. l, o Quantification of GFP-XVangl2 fluorescence around the cuticular plates (360°) of hair cells in neuromasts that show primI and primII polarity of wild type (l) and MZwnt11f mutants (o). p GFP fluorescence and Phalloidin enrichment (polarity ratio, calculated from the cap of max value to the lowest value) in the different conditions analyzed in (l, o). Error bars in l, o, and p represent S.E.M. ***Fisher’s exact test p < 0.01. Scale bar in a equals 5 μm, j equals 2 μm