Fig. 3.

LCOs retain the genetic characteristics of the original tissues after long-term cultured. a Heat-map analysis of the top 30 mutations in LCOs and their corresponding LC tissues. O LCO, T patient tissue. b Comparison of VAF of genetic alterations detected in LCOs and LC tissues. c Heatmap showing somatic mutations affecting cancer genes in LC tissues and paired LCOs. d Venn diagrams indicating the number of somatic mutations present in each LC tissues and their paired LCOs. e Heatmap showing somatic mutations affecting cancer genes in early passage and late passage organoids. f Venn diagrams indicating the number of somatic mutations present in early passage and late passage organoids