Figure 5.

Not only the interaction between JRAB-CH and filamin, but also JRAB ZF1 free from JRAB-C, is required for the function of open-form JRAB/MICAL-L2. (a) NIH3T3 cells expressing GFP‐JRAB WT or the indicated GFP‐JRAB variant were fixed and processed for rhodamine–phalloidin staining (red). Bar, 25 μm. Results shown are representative of at least three independent experiments. Schematic indicates the conformation of JRAB variants (right). (b) Cell lysates from HEK293 cells expressing GFP‐JRABΔZF1, ‐JRABΔCC, or ‐JRABΔCT together with HA‐filaminA were immunoprecipitated (IP) with anti‐HA antibody. Each immunoprecipitate was subjected to SDS‐PAGE, followed by WB with anti‐GFP and anti‐HA antibodies. Full-length blots with multiple exposures are presented in Supplementary Fig. S7.