Fig. 5.

Histological analysis of the brains of 40-day-old male mice. a Drawing illustrating the location where histological measurements were obtained. Coronal sections are marked in millimetres from the bregma according to the Mouse Brain atlas of [50]. Arrows and areas in colour correspond to thickness and area measurements to the following brain regions: primary motor cortex (M1) (light blue), corpus callosum (yellow, pink) and striatum (light green). Ventricles are labelled in black. b Representative images of cresyl violet staining performed on 20-μm-thick cryosections from P40 mice neonatally exposed to Vehicle + Normoxia or LPS + Hypoxia. No infarcts or lesions were observed. c Thickness (μm) and/or area measurements (μm²) of M1, corpus callosum and striatum regions, whole brain and ventricles. Bar graphs show means ± SEM (n = 6/group). Scale bars: 200 μm. Abbreviation: LPS lipopolysaccharide