Figure 4.

Combinational effect of ciprofloxacin and ivacaftor on Pseudomonas aeruginosa PAO-1 biofilms; 0.05 µg/mL ciprofloxacin and 8 and 16 µg/mL of ivacaftor significantly reduced biofilm mass in a synergistic fashion with ivacaftor. Statistically significant synergism was noticed between ciprofloxacin and 16 µg/mL of ivacaftor (P < .0001) in reducing PAO-1 biofilm formation. Asterisk represent significance compared to the control group (P < .05, 1-way ANOVA and Tukey’s test). Three different concentrations of ciprofloxacin were used: 0.01, 0.02, and 0.05 µg/mL. Within the same ciprofloxacin group, a statistical difference with a P < .05 among different ivacaftor groups was marked by a #. NS means no statistical significance. All groups consisted of at least 4 experiments.